FQ—PCR检测丹江口地区解脲支原体、沙眼衣原体的五年回顾性研究(1)
摘要：目的 分析研究实时荧光定量聚合酶链式反应(FQ-PCR)对解脲支原体、沙眼衣原体的检测结果，以了解本地区在不同性别及年龄段的感染情况。方法 选取1000例疑似非淋菌性尿道炎患者，均采取FQ-PCR进行检测，统计分析不同男女性别及年龄段的阳性检出率。结果 UU和Ct的检出率为44.50%，其中在女性患者中的检出率(55.31%)要明显高于男性(35.58%)(P<0.05)。其中女性患者UU的检出率(37.61%)显著高于男性(18.25%)，差异具有统计学意义(P<0.05)，而Ct和UU+Ct在不同性别间检出率无明显差异(P>0.05)。18～40岁患者的阳性率(81.35%)显著高于年龄大于40岁患者(18.65%)，差异显著(P<0.05)。结论 FQ-PCR对UU、Ct的检出率较高，本地区以女性患者及18～40岁年龄段的检出率较高，应加大对上述人群的监管力度。
關键词：FQ-PCR；解脲支原体；沙眼衣原体
A Retrospective Study of FQ-PCR in Danjiangkou Area of Ureaplasma Urealyticum and Chlamydia Trachomatis in Five Years
, 百拇医药
DENG Li1，SONG Dan1，FANG Zheng-chun2
(1.Department of Clinical Laboratory，The First Hospital of Danjiangkou City，Danjiangkou 442700，Hubei，China；2.Department of Anesthesiology，The First Hospital of Danjiangkou City，Danjiangkou 442700，Hubei，China)
Abstract：Objective Analysis of real-time fluorescence quantitative polymerase chain reaction (FQ-PCR)of Ureaplasma urealyticum and Chlamydia trachomatis detection results， in order to understand the region in different gender and age of infection. Methods A total of 1000 cases of suspected patients with nongonococcal urethritis， FQ-PCR detection. Statistical analysis of different gender and age of the positive rate. Results The detection rate of UU and Ct was 44.50%， among which the detection rate (55.31%) in female patients was significantly higher than that in males(35.58%)(P<0.05).The detection rate of UU(37.61%)in female patients was significantly higher than that in male (18.25%)， and the difference was statistically significant(P<0.05)，while there was no significant difference between UU+Ct and Ct (P>0.05). The positive rate (81.35%) of 18-40 years old patients was significantly higher than that in patients older than 40 years (18.65%)， and the difference was significant (P<0.05). Conclusion The detection rate of UU in FQ-PCR and Ct is higher， the detection rate of female patients in the local area and the age of 18～40 years old is higher， and the supervision of these people should be increased.
, 百拇医药
Key words：FQ-PCR；Ureaplasma urealyticum；Chlamydia trachomatis
在临床中，非淋菌性尿道炎(nongonococcalurethritis，NGU)属于常见性传播疾病[1-2]。该病在欧美等发达国家、地区的发病率居首位，且自2001年以来我国报道NGU发病率已经超过淋病，在全国监测8种性传播疾病中居首位。NGU及并发症，如睾丸炎、前列腺炎、盆腔炎、不孕不育等对人类的健康构成极大的危害。因此需要对该症进行及时诊断，以免耽误治疗[3-5]。而据统计在NGU中40%～70%的病例由解脲支原体(UU)和沙眼衣原体(Ct)引起。以前由于检测手段的限制，对UU和Ct检出率较低，无法及时治疗。近年来随着医学的不断进步，检测手段也随之进步，尤其是FQ-PCR技术的应用使得UU和Ct检出率不断增高，已成为临床检测UU和Ct的首先方法。为了解本地区5年来NGU的发病特点，本次研究选取FQ-PCR检测方法对我院在2009年1月～2014年12月收治的疑似NGU进行检测，结果如下。, 百拇医药(邓莉 宋丹 方正春)
關键词：FQ-PCR；解脲支原体；沙眼衣原体
A Retrospective Study of FQ-PCR in Danjiangkou Area of Ureaplasma Urealyticum and Chlamydia Trachomatis in Five Years
, 百拇医药
DENG Li1，SONG Dan1，FANG Zheng-chun2
(1.Department of Clinical Laboratory，The First Hospital of Danjiangkou City，Danjiangkou 442700，Hubei，China；2.Department of Anesthesiology，The First Hospital of Danjiangkou City，Danjiangkou 442700，Hubei，China)
Abstract：Objective Analysis of real-time fluorescence quantitative polymerase chain reaction (FQ-PCR)of Ureaplasma urealyticum and Chlamydia trachomatis detection results， in order to understand the region in different gender and age of infection. Methods A total of 1000 cases of suspected patients with nongonococcal urethritis， FQ-PCR detection. Statistical analysis of different gender and age of the positive rate. Results The detection rate of UU and Ct was 44.50%， among which the detection rate (55.31%) in female patients was significantly higher than that in males(35.58%)(P<0.05).The detection rate of UU(37.61%)in female patients was significantly higher than that in male (18.25%)， and the difference was statistically significant(P<0.05)，while there was no significant difference between UU+Ct and Ct (P>0.05). The positive rate (81.35%) of 18-40 years old patients was significantly higher than that in patients older than 40 years (18.65%)， and the difference was significant (P<0.05). Conclusion The detection rate of UU in FQ-PCR and Ct is higher， the detection rate of female patients in the local area and the age of 18～40 years old is higher， and the supervision of these people should be increased.
, 百拇医药
Key words：FQ-PCR；Ureaplasma urealyticum；Chlamydia trachomatis
在临床中，非淋菌性尿道炎(nongonococcalurethritis，NGU)属于常见性传播疾病[1-2]。该病在欧美等发达国家、地区的发病率居首位，且自2001年以来我国报道NGU发病率已经超过淋病，在全国监测8种性传播疾病中居首位。NGU及并发症，如睾丸炎、前列腺炎、盆腔炎、不孕不育等对人类的健康构成极大的危害。因此需要对该症进行及时诊断，以免耽误治疗[3-5]。而据统计在NGU中40%～70%的病例由解脲支原体(UU)和沙眼衣原体(Ct)引起。以前由于检测手段的限制，对UU和Ct检出率较低，无法及时治疗。近年来随着医学的不断进步，检测手段也随之进步，尤其是FQ-PCR技术的应用使得UU和Ct检出率不断增高，已成为临床检测UU和Ct的首先方法。为了解本地区5年来NGU的发病特点，本次研究选取FQ-PCR检测方法对我院在2009年1月～2014年12月收治的疑似NGU进行检测，结果如下。, 百拇医药(邓莉 宋丹 方正春)
参见：首页 > 医疗版 > 疾病专题 > 眼科 > 结膜病 > 结膜炎 > 衣原体性结膜炎(沙眼)